Pipeline

We are advancing a deep and diverse pipeline of therapeutic programs intended for rare diseases, such as genetic hypertrophic cardiomyopathy (gHCM) and genetic arrhythmogenic right ventricular cardiomyopathy (gARVC), as well as for more prevalent forms of heart disease such as dilated cardiomyopathy (DCM) and heart failure with preserved ejection fraction (HFpEF).

MYBPC3 Gene Therapy Program for Genetic HCM

We are developing an AAV-based gene therapy designed to deliver a functional MYBPC3 gene in adults and children with gHCM due to MYBPC3 gene mutations, estimated to affect more than 115,000 patients in the United States. These mutations can cause the heart walls of affected individuals to become significantly thickened, leading to fibrosis, abnormal heart rhythms, cardiac dysfunction, heart failure (HF), and sudden cardiac death in some adults and children. Based on publicly available information to date, we believe there are currently no approved treatments that address the underlying genetic cause of this disease.

Our product candidate, TN-201, uses a differentiated approach designed to enable robust expression of the MYBPC3 gene in the heart. We have demonstrated significant and durable disease reversal and survival benefit in a relevant murine model after a single dose, as well as tolerability in mice and non-human primates (NHPs).

The program is currently in IND-enabling studies, and we have obtained feedback from multiple regulatory agencies, including the FDA, to guide our path to clinical development. TN-201 has also been granted Orphan Drug Designation (ODD) by the FDA. We expect to submit an IND to the FDA in the second half of 2022.

MYBPC3 Program Publications & Presentations

PKP2 Program for Genetic ARVC

We are developing an AAV-based gene therapy designed to deliver a functional PKP2 gene in adults with gARVC due to PKP2 gene mutation, estimated to affect more than 70,000 patients in the United States. These mutations can cause enlargement of the right ventricle in affected individuals, replacement of heart muscle with fibrotic tissue and fatty deposits, and severely abnormal heart rhythms (arrhythmia) that can make it harder for the heart to function properly and result in sudden cardiac death in some adults and children. Based on publicly available information to date, we believe there are currently no approved treatments that address the underlying genetic cause of this disease.

With our product candidate, TN-401, we have demonstrated prevention and reversal of disease progression and survival benefit in a murine model after a single dose. Based on publicly available information to date, we believe these data are the first known demonstrations of durable disease modification, survival benefit, and prevention of arrhythmia using an AAV:PKP2 gene therapy construct.

We are currently initiating IND enabling studies for the program and expect to submit an IND in 2023.

DWORF Gene Therapy Program for Dilated Cardiomyopathy (DCM)

We are developing an AAV-based gene therapy designed to deliver the DWORF gene for patients with DCM, estimated to affect about one million patients in the United States. DCM is a progressive and life-threatening disease that causes left ventricle (LV) enlargement, LV wall thinning, insufficient contraction, reduced blood flow, ventricular arrhythmias, and can result in premature morbidity and need for heart transplant in affected individuals. DWORF is a muscle specific micro-peptide first discovered by our co-founder Eric Olson, Ph.D. that acts on the sarcoplasmic/endoplasmic reticulum Ca2+ ATPase 2a (SERCA2a) pathway, widely considered to be a promising target in HF.

We and our academic collaborators have accumulated significant preclinical in vivo proof-of-concept evidence for the therapeutic benefit of overexpression of the DWORF gene in multiple murine models, including models of gDCM and HFrEF, as well as tolerability in murine models. Based on publicly available information to date, we believe these are the first demonstrations of the potential benefit of AAV:DWORF.

This program is currently at the candidate selection stage.

DWORF Gene Therapy Program Publications & Presentations

TN-301: HDAC6 Inhibitor for HFpEF

We are initially developing TN-301, a first-in-class small molecule histone deacetylases (HDAC) 6 inhibitor for the potential treatment of heart failure with preserved ejection fraction (HFpEF). HFpEF is a common type of heart failure associated with aging, diabetes and obesity. It is estimated to affect more than three million people in the U.S. alone, and its prevalence is growing. HFpEF is characterized by a stiffening of the heart muscle resulting in an inability for the left ventricle to relax properly during normal heart rhythm, referred to as diastolic dysfunction. Symptoms initially include fatigue, shortness of breath and tissue swelling, resulting in reduced capacity for physical activity. Over time, this results in a substantial limitation in activities impacting quality of life. HFpEF patients are at risk of hospitalizations due to heart failure symptoms and premature death.

There are several cellular processes thought to underly the pathophysiology of HFpEF, including increases in fibrosis and inflammation as well as defects in metabolism.TN-301 was discovered using our proprietary Precision Medicine platform approach. TN-301 is a highly selective inhibitor of HDAC6, an enzyme in the cell cytoplasm that interacts with proteins, including tubulin, to coordinate cellular processes known to directly contribute to HFpEF. In preclinical studies, Tenaya’s HDAC6 inhibitors have been shown to increase cardiac function, reduce inflammation and fibrosis, reverse diastolic dysfunction and improve glucose tolerance, as well as achieve comparable in vivo efficacy to the SGLT2 inhibitor empagliflozin in a model of HFpEF.

Tenaya is currently enrolling healthy participants in a Phase 1 clinical study to evaluate the safety and tolerability of TN-301.

HDAC6 Inhibitor Publications & Presentations

Reprogramming Program for Heart Failure Due to Prior Myocardial Infarction (MI)

We are developing an AAV-based approach to cellular regeneration that involves converting (or reprogramming) existing cardiac fibroblasts (CFs) within the heart to turn into new cardiomyocytes (CMs) and to replace cells permanently lost due to MI. There are estimated to be more than four million patients in the United States living with HF due to prior MI. The loss of CMs in affected individuals permanently impairs heart contraction, leading to HF and potentially fatal arrhythmias, and the death of approximately 5% to 10% of MI survivors within the first year. There are currently no approved treatments that address the underlying loss of heart tissue.

The potential utility of our unique approach to creating new CMs was first demonstrated by our co-founder Deepak Srivastava, M.D. We have discovered a proprietary combination of three genes that can drive robust in vivo reprogramming of CFs to CMs when delivered together in a single AAV capsid. We have demonstrated significant and durable disease reversal as well as tolerability in multiple small and large animal models. Based on publicly available information to date, we believe our results in a pig model of HF due to prior MI represent the first-ever successful demonstration of the potential beneficial effect of this approach in a human-sized heart.

This program is currently at candidate selection stage.

Reprogramming Publications and Presentations